The aim of the present work was to study the biochemical constituents of four species of Cyanobacteria isolated from paddy fields of Warangal district. The biochemical constituents were analyzed in terms of chl-a, carotenoids, total proteins, total carbohydrates and phycobiliproteins content of four Cyanobacterial species, Anabaena circinalis, Nostoc punctiforme, Oscillatoria princeps and Phormidium mucosum were analysed. The analysis showed that maximum chlorophyll-a content in A.circinalis (2.52±0.06) and minimum in P. mucosum (1.23±0.43), whereas the maximum carotenoids content in O.princeps (1.57±0.01) and minimum in A.circinalis (0.98±0.14), the maximum amount of total protein in N.punctiforme (2.89±0.09) and minimum in P. mucosum (1.98±0.07), also maximum amount of total carbohydrate in A.circinalis (3.21±0.25) and minimum in P. mucosum (2.07±0.09), whereas the maximum Phycocyanin content in O.princeps (5.08±0.12) and minimum in P. mucosum (3.45±0.02), the highest allophycocyanin content in P. mucosum (3.94±0.51) and lowest in O.princeps (1.05±0.01), and the maximum Phycoerythrin content in N.punctiforme (3.56±0.71) and minimum in O.princeps (1.02±0.57) respectively. The results of this experiment revealed that in food, pharmaceutical, cosmetics industries and biotechnological applications.