Nanobiotechnology, a new exploration gives rise to the emerging future of science bygone decade. It covers enormous technical branches like biotechnology, chemical, medical and robotic engineering. Silver and silver-constructed product influence burly bacterial and fungal activity. In the present work, a study which compares the synthesis of silver nanoparticles (AgNPs) from E.coli using two different media, LB and M9 medium respectively. The influence of the parameters kind of time, substrate concentration and pH on the synthesis of nanoparticles were studied.The synthesized nanoparticles were characterized and showed antimicrobial on well diffusion assay.
