The present study indicated higher shoot and root length, dry matter, yield and alkaloid content of ashwagandha, when the mixed inoculant of Azospirillum, Azotobacter, Bacillus and Pseudomonas was applied. The free-living plant growth-promoting rhizobacteria (PGPR) can be used in a variety of ways to increase the plant growth. The addition of PGPR increased the germination rate, root growth, leaf area, chlorophyll content, magnesium content, nitrogen content, protein content, hydraulic activity, tolerance to drought, shoot and root weights, and delayed leaf senescence which reflected in higher grain yield.
