A Micropropagation protocol is presented for the conservation of critically treated plant species. The present work was carried out in the different types of concentration with two basal media (MS & B5) and undertaken to develop a basic and simple protocol for shoot induction via. micropropagation of Ruta graveolens L., the best result is shown in MS media. The effect of two types of media on the behavior of in vitro connective micropropagation protocol was developed. Two media were tested B5 and Ms with different growth hormones for induction stages. MS medium is to be the best medium for plant tissue culture, especially shoot induction. High frequency of induced shoot was obtained on BAP (2 mg/l) KN(1.5mg/l) and NAA(1mg/l). Very low concentration of growth hormones did not support in vitro shoot induction in Ruta graveolens L. The plant tissue culture methods provide unique protocol and developed in the biodiversity conservation and for the further investigation further work for standardized of efficient in vitro protocol for the best shoot multiplication in and in - vitro Rooting in under progress in our laboratory.
