A micropropagation protocol is presented for the conservation of critically threatened woody tree species. The present work was carried out in different types of media and undertaken to develop a basic and simple protocol for shoot induction via micropropagation of Saraca indica L. Best result shown in different media in the order as WPM>MS>B5 media. The effect of different mediums on the behavior of in-vitro consecutive micropropagation protocol was developed. Three media were tested (B5 MS, and WPM) with different growth hormones for the induction stage. WPM was found to be the best medium for shoot induction. A high frequency of induced shoots was obtained on BAP (6mg/l), KN 1mg/l), and NAA (1.5 mg/l). The low concentrations of growth hormones did no support in vitro shoot induction in Saraca India L. The shoot induction protocol developed in this study provides a basis for germplasm conservation and for further investigation of medicinally active constituents of the elite medicinal plant. Further work for standardization of efficient in-vitro protocol for best shoot multiplication and in-vitro rooting is under progress in our laboratory.
