A micropropagation protocol is established for conservation of critically threatened wood tree species Mallotus philippenesis. The indirect shoot regeneration from leaf explants protocol developed in this study provides a basis for germplasm conservation and further investigation of medicinally active constituents of the elite medicinal plant. In the present experiment different explants such as leaves, cotyledons, root and in-vitro raised vegetative parts were used for callus induction. Leaves found to be the best explants for indirect shoot regeneration. MS media supplemented with 2-4D, BAP, KI responded best for callus formation. At higher concentration of growth hormones negative response was also observed. Indirect shoot organogenesis was achieved from the callus using BAP and 2-4D when highest number of shoots (2.0 ± 0.33) with maximum frequency (49.0±0.88) was regenerated. All the in- vitro raised shoots with a maximum length of (5.8±0.35) were transferred to rooting medium supplemented with different concentrations of auxins such as IBA and NAA. The best rooting response was observed on 1.0mg/l IBA with 1.0mg/l NAA. The well rooted plantlets were transferred to plastic cups containing soil+vermiculite + sand in 1: 1:1 ratio for hardening. Finally the hardened plantlets were transferred to field conditions for maximum survivability.
