Nanotechnology has emerged as an exciting approach in the drug development process and among the various nanoparticles, gold nanoparticles have been explored for its variety of medical applications. The present study demonstrates the in-vitro and in-vivo anti-inflammatory activity of biogenic gold nanoparticles using indole-3-carbinol (AuNPI3Cs). The anti-inflammatory activity of AuNPI3Cs was evaluated by in vitro methods and in vivo study were performed by carrageenan induced mice paw edema model to evaluate acute inflammation. AuNPI3Cs exhibited dose-dependent human red blood cell membrane stabilizing activity and prompted the inhibition of protein denaturation which was comparable to the standard anti-inflammatory drug, diclofenac sodium. In vivo study revealed that AuNPI3Cs significantly reduced mice paw edema after the induction of acute inflammation. The findings suggest that the synthesized gold nanoparticles from indole-3-carbinol could be a potent anti-inflammatory agent.
