Gonadotropic cells are distributed in the proximal pars distalis (PPD) of the pituitary in Notopterus notopterus and to know the structure by different age group using histochemical and electron microscopic techniques are applied for the current investigation. The PPD of pituitary gland in zero age group fish has very little number of gonadotropes. The cells are undifferentiated with indistinct boundaries. They markedly increased in number in one year aged fish, since the gonads of this age group fish shows advanced stages of growth. The gonodotropes that largely occupy the medial aspect of the PPD increased numerically as the gonads mature and become major cell type before spawning in two age group fish. The electron microscopic studies reveal that, they appear oval or elongated containing a few large granules and many small lucent secretory granules. Only one gonadotropic cell type could be identified in the PPD, although two similar cells containing different types of secretory granules are noticed. The differentiation and presence of two types of GTH needs further verification in the fish N. notopterus.
