One of the most important strategies towards the biological control of several phytopathogenic fungi is the application of Trichoderma species for as a possible eco-friendly pesticide. The secretion of chitinase breakdown the chitin cell wall of most of the fungal pathogens. Chitinase hydrolyses the glycosidic bonds of the fungal cell wall and weakens the pathogen. The present study shows that the Trichoderma spp. can be easily isolated from the soil and have different chitinase activities against fungal pathogens. In this study, three Trichoderma species were isolated (harzianum, Polysporum and viride) and their Chityinase assay were done to check their Chitinase wall degradation potential accordingly. The maximum amount of protein (486 mg) in the incubated broth was revealed for the isolate SIT2 i.e. for Trichoderma polysporum and least ( 265mg) for the isolate SIT5 i.e. for Trichoderma viride. The total chitinase activity were found to be highest (62.56 µmolmin-1) for T. harzianum isolate (SIT1) and least (21.36 µmolmin-1) for the T. polysporum isolate (SIT2). Furthermore, it was revealed that the specific activity of Trichoderma harzianum (SIT1) was maximum (0.190 µmolmin-1mg-1) and Trichoderma polysporum (SIT2) as minimum (0.0 43µmolmin-1mg-1).
