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Multiple metal resistances and its correlation to antibiotic resistance in bacteria isolated from industrial effluent

Author: 
Juhi Sharma, Shikha Bansal, Shanu Patial, Shivani Singh and Preeti Bala
Abstract: 

The different microorganisms such as bacteria, fungus has ability to treat water contaminated with heavy metal. The study carried out for heavy metal, removed by bacteria isolated from infected area to treat contaminated water collected from effluent of industries. Characterization of isolated bacteria showed resistance to heavy metal. The total 73 bacterial isolate were screened, using LB agar. Three resistant bacteria were selected using different agar media containing different concentration of Ar, Cu, and Zn. These bacteria showed optimum growth when incubated for 24 hour at 30 ̊c. The isolates were further examined using different heavy metal concentration. The selected organisms which show higher resistance to metal was identified as Bacillus, Staphylococcus and Klebsiella .antibiotic resistance of heavy metal tolerance was analyzed in detail showing resistance against Ampicillin and ciprofloxacin and minimum inhibitory concentration at 100µg/ml and 120µg/ml for Klebsiella and Staphylococcus respectively. This result reveals that, the bacterial strain shoes remarkable tolerance against heavy metal could be potent agent for the development of soil inoculum applicable in bio-augmentation of heavy metal sites.

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