My work is to study the life cycle of rust fungi in pearl millet. Histopathology is the study of the structural manifestations of diseases at the light microscopic level and it is the microscopic examination of biological tissues to observe the appearance of diseased cells and tissues in very fine detail. The study describes an improved method for fixation of sampling of fungal infected plant parts, staining and observation of fungal infections in plant tissue for histopathological visualization. During the study it was observed that in particular technologies such as stereo microscopy and compound microscopy have enhanced our ability to visualize hyphae in the plant tissue. We have done germination test to determine the percent germination and vigor index of seeds. Hemocytometer count has been done to determine the number of spores per ml which is used to spray on the young plantlets by brush method. Micrometry is also done to measure the dimensions of the rust spores. In order to study the host and pathogen interactions I have done maceration technique, and observed the entry of pathogen by the formation of haustoria.And also we noticed that a single species of rust fungi may be able infect two different host plants in different stages of its life cycle, and may produce up to five morphologically and cytologically distinct spore producing structures.
