The present investigation carried out on production of cellulase from agrowaste by Acinetobacter species isolated from clinical specimens. 4 Clinical samples collected from different labs, out of four clinical specimens, only one sample showed the presence of Acinetobacter species after culturing it on the Leeds Acinetobacter Agar Base medium and Blood Agar medium. Agrowaste such as wheat husk, gram husk and tuwar husk were collected for the production of cellulase. In the contemporaneous exploration after the incubation of 48 hours at 37oC it was resolute that the mixed substrate is appropriate for the production of cellulase which showed highest activity of cellulase 0.93nm at the concentration of 4600 micro gm/ mL of matose/ mL of enzyme per minute. However, the single substrate, Gram husk found to be most suitable among wheat husk, tuwar (Pigeon pea) husk for the production of cellulase. The optimum pH for the production of cellulase is found to be 7.0 and the optimum temperature was determined 40oC with 0.66 nm cellulase activity.
