Spodoptera spp. is a serious pest of several legumes, cotton and other cash crops. Lot of destruction of crop is attributed to this pest worldwide. This pest had become resistant to variety of pesticides, resulting in burden on economy and use of pesticides is well known for health hazards to human beings. In the current study attempt has been made to identify plant protease inhibitors which can inhibit the digestive protease of Spodoptera to develop a strategy to control crop plants from pests by introducing a transgene which is responsible for the inhibition. Spodoptera gut proteases inhibited by Piper cubeba, Mucuna pruriens 56.84% and 56.59%. respectively. Pimpinella anisum, Citrullus colocynthis, Juniperus communis,Piper cubeba, Apium leptophyllum, Cassia absus, Ipomoea hederacea Jacq. and Dryptes roxburghii could inhibit them partially. Trypsin inhibitory potential of different seeds was also carried out which shows Mesua Ferrea 387.2 ± 5.91TIU, Mucuna pruriens 4.9±3.44 was lowest. Protein content in seeds revealed Piper longum 0.432±0.22 g/g and Lepidium iberis 0.775±2.21g/g.
