Chilli (Capsicum annuum.L) is one of the most important commercial crops belonging to the family solanaceae. Which is grown throughout the tropical and subtropical parts of the world. Agriculture is heavily dependent on the use of chemical fertilizers and pesticides to achieve higher yields. This dependence is associated with problems such as environment pollution, health hazards, destruction of biological communities that otherwise support crop production. The overcome this problem, biofertilizer is the best source to replace the chemical fertilizer. In this study, five locations were selected in cuddalore district, tamil nadu. To isolate the Azospirillumand designated as AZS-1 to AZS-5. This Azospirillum isolates has capacity to produce IAA and screening for efficiency fixed Nitrogen by utilizing malic acid. The growth and yield parameter such as the highest plant height (78.60 cm), The maximum number of fruits (46.50) per plant, The highest fruit yield (208.26g) per plant. To efficient strains selected and treated with chilli var k-1 among the five treatment the plant growth recorded most excellent in T5 (75% RDF + Azospirillum sp).
