Research on utilization of agro waste as raw materials for the production of industrially important products are gaining importance these days. Present study was focused on exploring the abilities of laboratory isolates of Trichoderma viride to produce a value added product, tannase by utilizing agro wastes such as Guava leaves, Eucalyptus leaves ,Rice husk and Sorghum husk. In a submerged fermentation system. To achieve this goal, an in vitro investigation was carried out to assess the enzyme production by the efficient tannolytic fungus Trichoderma viride isolated from tea waste decomposed soil. Among the number of mycoflora Trichoderma viride showed maximum hydrolyzing zone for tannolytic activity, it was selected as a candidate for enzyme study. Among the different agro wastes as substrates in different concentration (1%, 2%, 3% and 4%) maximum tannase activity of 4.34±0.004 Umg-1 protein was observed in 3% Guava leaves. The least tannase activity of 0.06±0.005 Umg-1 protein was recorded at 1% Eucalyptus leaves.
