Zinc oxide nanoparticles are non-toxic, non hygroscopic polar inorganic and inexpensive material. Zinc oxide nanoparticles (ZnO) was successfully synthesized using zinc sulphate in Emblica officinalis, Terminalia chebula, Terminalia bellirica and Triphala extracts and then characterized. The antioxidant test was analyzed by Molyneux method using the strong antioxidant using DPPH method. In-vitro antidiabetic test was carried using alpha amylase and Glycation test. Alpha amylase is the breakdown of long chains of carbohydrates thus preventing the increase in blood glucose level. Glycation is the binding of sugar molecule to a protein or lipid molecules without enzymatic activity. It impairs or destroys the functioning of biomolecules. Nanoparticles annihilate cancer cells by flow and penetration to distinct regions of tumors through blood vessels and then driving to interstitial space to reach the target cells. ZnO nanoparticles exhibit highest percentage of antioxidant and in-vitro antidiabetic effect in alpha amylase and glycation test synthesized using Emblica officinalis, Terminalia chebula, Terminalia bellirica and Triphala extract. Triphala showed anticancer effect on MCF-7 cell line.
