Staphylococcus aureus has been reported to be a major cause of hospital acquired infections. Indiscriminate use of antibiotics resulted in the development of multi-drug resistant S. aureus throughout the world. In present study twenty strains of S. aureus were isolated from hospital waste samples like cotton swabs, bandages, and needle. These isolates were identified by several morphological and biochemical test. Antibiotic susceptibility test was performed by Standard disc diffusion method. All the isolates were found to be 100% resistant to Penicillin G (P), Cefoxitin (CX), and Erythromycin (E). The resistance pattern of reference strain S.aureus ATCC 25923 was 0%.
