Utilization of agro wastes and by products for the production of organic acid increasing worldwide. The demand for citric acid is increasing due to their wide application in the various industries like food, beverage, pharmaceutical and cosmetic. The present study was aimed to utilize mango peel as a substrate for the production of citric acid using Aspergillus niger isolates. Among the 6 isolates obtained from the fruit waste dumped soil,the isolate Aspergillus niger. I recorded highest yield of citric acid (4.52%).