Body fluids are the common and important type of forensic evidence. In particular the identification of Menstrual (liquid or stain) is often a key step during the investigation of rape case. This paper presents a method which is simple and easy for the differentiating menstrual blood from normal blood through its composition. In this microscopic analysis, hemoglobin content, RBC and WBC count was done as a preliminary study to distinguish the type of blood samples. The attempt has been made to determine the blood group from liquid menstrual blood by using antiserum ABH. Forensic analysis was done by performing confirmatory test of normal blood using Teichmann reagent which gives negative result with menstrual blood can be a good parameter for differentiating the menstrual blood from normal blood.