The present study was conducted to isolate, optimize and characterize the proteases from two leguminous seeds, Green gram (Vigna radiata) and Soybean (Glycine max). Isolation of proteases was performed by using Ammonium sulphate precipitation and then it was dialysed. The protein concentration of enzyme was 52µg & 69µg. The Optimum PH was found to be 9 and optimum temp. was 500C. The enzyme activity of soybean proteases and Green gram proteases was found to be o.16µg/ml/min and 0.23 µg/ml/min respectively. In Machaleis menten graph Km was found 0.485 & Vmax was 1.01. In Line weaver Burk Plot Km was found 5.55 & Vmax was 2. The Casein & Heamoglobin assays were also performed to check its catalytic activity. Molecular weight of proteases according to SDS-PAGE found to be 26.0KD Soybean & 19.0KD Green gram. FT-IR characterization was also done. The paper concludes that leguminous seeds can be source of proteases for industrial purposes.
