Water is and has always been a prized but scarce commodity. It is one of the most vital natural wealth for all life on earth. The present investigation is an attempt to know the pollution load through diatom as water pollution indicators, species diversity and their seasonal fluctuations in lake Ecosystem of four lakes of K.R.Nagar Taluk, Mysore District. Anthropogenic eutrophication, habitat destruction and water flow alterations were the key stressors to freshwater, threatening the quality and availability of water and biodiversity. Overall identified diatoms in four lakes are predicted as water quality indicators. Synedra ulna and Synedra acus were proved to be anthropogenic indicators in Meluru lake 1. In Meluru lake 2 Navicula halophila was responsible for organic pollution. Karpuravalli lake was dominated by Stauroneis phoeicenteron indicating anthropogenic activity. Saligrama lake was contaminated by Synedra acus and Navicula halophila causing both anthropogenic and organic pollution indicators. Results of the present study cautions the indiscriminate dumping and discharge of pollutants into the lakes which may lead to serious environmental deterioration which could be considered as a potential source of threat to biotic life.