Mulberry is a perennial plant that maintains high heterozygosity due to the out breeding reproductive system. It is recalcitrant to most of the conventional breeding methods to overcome of this problem non-conventional breeding methods such as polyploidy, endosperm culture, micro propagation and induction of mutations too have some long- range advantages such as showing better performance over the original parental characters with passage of time and for the production of good qualitative and quantitative characteristics of mulberry leaf along with the vision of overcoming biotic and abiotic stress. In- vitro techniques such as tissue and organ culture offer the plant breeder new openings in the colonel propagation, genetic manipulation and production of homozygous inbred lines to improve the nutritive value in Mulberry leaves.
