Comparison between results reported about antibacterial effects of different essences is very difficult, that differences in various methods for evaluating of antibacterial effects of different essences, resources of essences and different genus of used bacteria are from its reasons. Various studies has been performed about antibacterial effects of essences of herbal belong to Lamiaceae family and some of important compound in essences of this family among Carvacrol and Thymol (Almas, 1999). In present study, these essences are used against test bacteria, which were isolated from GB patients (Cholecystitis, Cholangitis, GBC).