Satyrium nepalense D. Don. belonging to family Orchidaceae is a highly valuable ethnomedicinal herb. Its toxicity study was not conducted earlier. So, acute oral toxicity of tuber extract of Satyrium nepalense D. Don. was assessed in albino Wistar rats with a single dose of the extract at 300mg/ Kg body weight and 2000 mg/Kg body weight. Mortality/viability and clinical signs were recorded on test day 0 (prior to administration), after 24 hours of drug administration, on test days 7 and 14. Rats showed no clinical signs of toxicity and no gross pathological changes. All animals appeared normal throughout the experimental procedure. The seed extract was found non-toxic to rats and helped in weight gain with LD> 2000 mg/Kg body weight. The conclusion drawn from this study specify that oral administration of Satyrium nepalense is not connected with any toxicologically significant effects and the data could provide satisfactory preclinical evidence of safety to launch a clinical trial on a standardized formulation of the plant extracts.
