The prevalence of bacteria on computer keyboards in cyber cafes situated in and around Rivers State University of Science and Technology, Port Harcourt, Nigeria was investigated. A total of 200 computer keyboards were examined by culture methods for the presence of bacteria. Out of the 200 computer keyboards, 40 each were taken from the 4 cyber cafes including the university Information and Communication Centre (ICT). The numbers examined were: FCB 36(18.0%) had bacteria growth, SPC 20(10.0%), BB 30(15.0%), RGBC 34(17.0%), ICT-RSUST 30(15.0%) respectively. From the results, 58(36.6%) had 1-5 counts, 78(52.6%) had counts from 6-10 counts and 13(8.6%) had counts ranging from 10 and above colonies. The overall percentage occurrences of isolated bacteria were: coagulase-negative Staphylococci 116(55.8%), Staphylococcus aureus 42 (20.2%), Bacillus spp. 24(11.5%) and Escherichia coli 26(12.5%). Overall prevalence of bacteria from the cyber cafes were FCB 78(37.5%), SPC 38(18.2%), BB 28(13.4%), RGBC 30(14.4%) and ICT-RSUST 34(16.3%). FCB had the highest prevalence of bacteria and coagulase-negative Staphylococci were highest in percentage occurrences.
