The microbial fuel cell (MFC) technology has captured the attention of the scientific community for the possibility of transforming organic waste directly into electricity though microbial catalyzed anodic and cathodic electro chemical reaction. The aim of the study is to determine the most efficient wastewater source that can generate the highest rate of electricity production by using MFCs and to determine the removal rate of carbon and nitrogen in wastewater by using MFCs. A two chamber MFCs process is developed for the treatment. The wastewater sample used was activated sludge. The highest rate of volts generation is achieved when the MFC was operated with in Saccharomyces cerevicea, (0.560v) and staphaylococcus aureus (0.450v) was produced in wastewater.