Human activities disturb the diversity and distribution of all creatures in any region. The main aim of this study is to know the effects of human activities on the diversity of butterflies in three of the main sites of Seshachalam hills and Nallamala hills in Eastern Ghats, Andhra Pradesh, India. The diversity and composition of butterflies varied in all the three study sites showing the effect of temperature and disturbances associated with other climatic and ecological parameters. In the present study, a total of 564 individuals belonging to 82 species of five families were identified. Among which Nymphalids was found to be the most dominant family with 223 individuals and constituting 39.13% of the total abundance and the most dominant family in terms of species richness having 58 species. Shannon wiener diversity index, abundance and species richness were found to be maximum for the undisturbed site and minimum for the disturbed area.