A total 9 species belonging to 5 genera of fungi were isolated from rhizospheric soil of Canna indica L. from botanical garden of Yeshwant Mahavidalaya, Nanded during December 2017. The mycoflora were isolated by using soil dilution method on Potato Dextrose Agar medium supplemented by suitable antibiotics such as streptomycin. The identification and characterization of the mycoflora were completed with the help of authentic manuals of fungi. The most common fungi among them viz; Aspergillus niger, Aspergillus flavus, Aspergillus nidulans, Penicillium frequentans, Penicillium chrysogenum, Trichoderma viride, Fusarium oxysporum and Fusarium solani were isolated and characterized. The Aspergillus niger and Aspergillus flavus has dominant fungal species. The percentage frequency of the mycoflora was analyzed.