Diet used by an organism provides energy which is required for various activities like, locating food, mate, escape from predators, defense of territories and response to stress. In the present study flies reared in natural and synthetic energy drinks were used to study the effect on locomotor ability so it was found out that feeding rate was found to be greater in flies reared in natural energy drinks compared to flies reared in synthetic energy drinks as well as normal media, Further it was also noticed that larval crawling and adult climbing ability was found to be significantly greater in flies reared in natural energy drinks, compared to synthetic and normal, Thus these studies suggests that natural energy drinks had beneficial effect compared to synthetic energy drinks and it may provide greater energy.