An investigation was undertaken to study the effect of graded levels of nitrogen and Azospirillum on yield and quality of ash gourd (Benincasa hispida Cogn.) cv. CO.1 in the Vegetable unit, Annamalai University, Annamalai nagar during January-May 2016. The doses comprised of 5 levels of nitrogen viz., 30, 45, 60, 75 and 90 kg ha 1 and in two levels of Azospirillum {with and without Azospirillum (A1 and A0 respectively)} with a total of 10 treatments replicated thrice in a randomized block design (RBD). The results revealed that the treatment of combined application of nitrogen 75 kg ha-1 along with Azospirillum @ 2 kg ha-1 of soil application recorded the highest number of fruits per vine, fruit length, fruit weight and fruit yield per plot and NPK uptake.