The indigenous systems of medicine namely Siddha, Ayurveda and Unani have been in existence for several centuries. The major lacuna in the cultivation of Withania somnifera is non-availability of standard agro techniques to increase the yield and quality. It is obvious that without clear understanding on the cultivation Withania somnifera of, the use of various chemicals and in organics may deteriorate the quality of the products. Novel solutions for plant growth enhancement are required to increase the yield and quality of and to reduce the Withania somnifera burden on our resources and environment. A pot culture experiment was conducted Department ofl Microbiology Sribharathi Arts and Science College for Women, Kaikkurichi, Pudukkottai, Tamilnadu, India., to know the effects of inoculants, diazotrophs like Azospirillum, and Azotobacter on seed germination and vigour index of Withania somnifera.