Introduction: This retrospective study aims to determine the prevalence and resistance pattern of Acinetobacter baumannii in clinical isolates from a private hospital in Kolkata, Eastern India. Methods: The study was conducted over a 6 months period from October 2017 to March 2018 where all the clinical isolates of A. baumannii and their resistance pattern obtained by an automated identification system (Vitek 2 Compact). Results: Out of 2149 clinical samples, a total of 21 strains of A. baumannii were isolated. Majority of isolates (62%) were obtained from ICU followed by IPD (28.5%) and OPD (9.5%). Majority of samples were from tracheal aspirates (43%) and urine (28.5%). Patients of more than 61 years of age are affected most. Out of twenty one strains four were found as Multi Drug Resistant (MDR) and fourteen were Extensive Drug Resistant (XDR) strains. Predominant MDR strains were from tracheal aspirates. 90% strains were resistant to Ciprofloxacin and Levofloxacin. The strains were sensitive to only Colistin and Polymixin-B. Conclusion: It may be concluded from the study that XDR strains are more predominant than MDR strains of A. baumannii among the clinical isolates of this private hospital. This epidemiological study will help to conduct better infection control policy and resistance pattern guide for the treatment of patients in this region
