Background: Immunoglobulin Y (IgY) found in egg yolks performs similarly to mammalian IgG, but with distinct physicochemical characteristics and biological activity. An innovative form of immunotherapy with Immunoglobulin Y is used to promote passive immunity and is regarded as a secure substitute for antibiotics in the treatment of periodontitis. Aim: A study was conducted to determine the minimum inhibitory concentration (MIC) and zone of inhibition (ZOI) of Ig Y against P.gingivalis. Material and Methods: P.gingivalis from stock bacteria was cultured and then incubated for 18 - 24 hours at 37°C. Colonies were added to 3 ml of liquid Brain Heart Infusion media, and they were then incubated at 37 °C for 18 hours. The turbidity of 0.5 McFarland standards, which is equal to 108 CFU/ml, was used to compare the bacterial suspension in BHI media. IgY that had been extracted and purified was tested at concentrations ranging from 5 mg/ml to 50 mg/ml. IgY was added to culture plates, and a clear zone—a sign that the addition had inhibited bacterial growth—was noticed. Results: The antimicrobial activity against P.gingivalis with a ZOI of 13-14mm at 320µg/mL was noted. MIC showed promising results above 360µg/mL 50% reduction and 380µg/mL 90% reduction. Conclusion: A Concentration of 320µg/mL of IgY is required to cause an inhibitory effect on P.gingivalis. IgY as LDD or as an adjunct to SRP can have a promising effect on the healing of periodontal diseases.
