The present study deals with the GC-MS analysis of one Ayurvedic formulation Gandharvahasta-Taila. This preparation is used to treat many neurological as well as inflammatory disorders in Ayurveda. The GC-MS analysis results indicated the presence of several biomolecules of which some of them like Eicosatrienoic acid, palmitic acid, ricinolic acid, stearidienoic acid, linolic acid and oleic acid were present in higher quantities. The study tries to correlate the medicinal activity of the medicine with the some important biomolecules known for their similar medicinal roles.