Walsura is a plant genus of the family Meliaceae. Walsura have high medicinal importance and theselected species W. trifoliolatais used traditionally to treat various diseases. In the present study was undertaken to analyze the chemical composition, the crude methanolic leaf extract of W. trifoliolata was subjected to phytochemical screening for the presence of various phytochemicals. About 22different compounds were identified using GC-MS. From the present findings Lupenol an active phytocompound identified and it is having the qualities of anti-inflammatory, anti-diarrheal and anti-cancer and correlated with earlier studies. Isolation of Lupenol from the W.trifoliolata which were fascinating manyscientists to work on this group of plants.