Background: Urinary tract infection (UTI) is a health problem of mankind, common among women during pregnancy, and is one of the leading causes of miscarriages, premature births, and underdevelopment of infants. This study aimed to isolate and identify the bacteria species present in the urine of pregnant women attending ante-natal clinic in Federal Medical Centre, Owo, Ondo State. The antibiotic resistant patterns of such isolate(s) was examined. Methods: In this study, descriptive analysis and chi square at P>0.05 and 95% confidence interval was used.One hundred(100) urine samples of pregnant women were collected in sterie universal containers, then cultured on blood agar and MacConkey agar using standard procedure. Results: Results revealed a total of 72% prevalence. Of bacterial infection. The age range of 26-30 years had the highest prevalence of UTI (36%) and age range (20-25) had the least prevalence of UTI (7%). Escherichia coli (43.06%) was the most prominent as the cause of infection among pregnant women, followed by Klebsiella spp. (25%), Staphylococcus aureus, (18.06%), and Pseudomonas aeruginosa (13.88%). Ofloxacin and augmentin have the highest resistance to all bacteria isolated while cefuroxime, gentamycin, and imipenem showed least resistance to all the bacteria isolated. Conclusion: Antibiotic resistance remains a major problem among the populace around this part of the World probably due to antibiotic abuse and misuse. Good personal hygiene will go a long way in reducing the incidence of UTI among the populace especially in pregnant women and should be a regular feature in antenatal care awareness campaign.
