The present study investigates the lichen species identification by using morphological and chemical analysis. There were six different lichen species were collected identified based on different standard procedures and analyzed for its bio-potentials. Anatomically, they were examined for their growth type and thallus color. Spot test, and micro-crystallography were analyzed, and chemical analysis were applied to lichen fragment and their extracts for the species identification. To characterize the lichen in terms of secondary metabolites. The results showed the presence of several types of phenolic compounds; namely tannins, flavonoids and other secondary metabolites such as alkaloids, sterols and terpenes, essential oils, saponins and quinones. Thin Layer Chromatography (TLC) was performed to confirm the qualitative characterization of photochemical. To separate different molecules of each secondary metabolite showed the diversity of metabolite in extracts. The TLC result was reported in the form of spots and frontal report was expressed in Rf value. It reflects the purity of the component molecules in lichen. This diversity of secondary metabolites can be at the origin of the widespread medicinal properties and therapeutic uses of the tested lichen.
