The present study is focused on the extraction of intracellular pigment from actinobacteria isolated from different areas of Aurangabad, Maharashtra, India. A total 7 Actinomycetes strains were isolated, out of a total seven isolates, five actinobacterial isolates showed pigmented growth. The biochemical characterization of isolates was also studied. Different production media were used for pigment production and MGYP was selected for large scale production. Further the intracellular pigment was extracted by cell disruption technique using ultrasonication and partially purified using ethyl acetate as the solvent. Partial characterizations of the pigments were carried out using UV-Visible spectrophotometry. The extracted pigment showed significant antibacterial activity against Gram negative and Gram positive bacteria. The obtained results showed broad spectrum antibacterial activity.
