Banana fruit is found to be one of the essential source for all the nutrient content in it. Due to increase in the population the demand for the food crops increases. Microprapagation in invitro condition is one of the best methods adapted to increase the productivity. In this study suckers from the matured banana plant was taken and surface sterilized and media preparation was done using different composition of macromolecules, micro molecules and trace elements for the multiplication of propagated plantlets in the MS medium this step was followed by the standardization of photoperiod for the micro propagation. Medium containing BAP, Ascorbic acid and agar composition gave a good result and the shoot induced plantlets were transferred to the root inducing medium were standardization of root inducing medium was done with different composition of charcoal in which charcoal containing 2% charcoal gave a good yield after which the plantlets were transferred to the primary hardening stage. In this stage we can eliminate the poorly developed plants and the diseased plantlets.
