Botulism intoxication in bovine caused by exposure to botulinum neurotoxins (BoNTs), one of the most potent toxins known, produced by the bacteria Clostridium botulinum. It can affect all mammals. Recent years, an increased frequency of new form of bovine botulism has been observed. A total of 121 Cattles and 10 sheep and goats irrespective of breed, age and sex included in this study. Cultural examination of rumen fluid, dung samples and poultry manure samples, only three cases showed photolytic changes in cooked meat medium and lecithenase activity in Clostridium botulinum isolation agar. The positive bacterial culture showed sub terminal spore in gram staining, acid- gas production in carbohydrate fermentation in lactose and sucrose medium, liquefaction of gelatin and stormy clot fermentation in milk. In case PCR reaction, two samples showed 800bp product size in 2% agarose gel.
