Berberine has long been used as a traditional medicine because of its potential activity against Bacterial, fungal, viral diseases. Berberine have a complex and diverse chemical structure provide a base for different biological targets. The present study deals with the novel encapsulation of biopolymer called Collagen1 with berberine by Electrospinning method in different compositions, parameters, and method for preparation. By electrospinning not only we get desired mechanical and biological properties of encapsulation studies, but also used to combine these two different materials with wide range of drug delivery science and tissue engineering properties to produce electrospun nanofibres at different compositions. The physical and thermal properties of electro spun nanofibres interaction and were examines under FE-SEM. resultant studies may be used in variety of applications including Chemical, Genetical, Immunological and industrial purposes.