Aspergillus species is one of the most suitable species to grow in the solid substrate for maximum glucoamylase production. The enzyme titres varied considerably in different substrates. The particle size and the chemical composition of the substrate influence the fungal growth and enzyme production. The aim of our study is to analyze the biochemical contents present in the selected substrates (Rice bran, wheat bran and tea waste) for glycoamylase production and to optimize the moisture content in the selected substrates to enhance glucoamylase production and also to find out the effect of various physical and biochemical parameters to maximize glucoamylase production. From the study it was found that good production of glucoamylase from Aspergillus niger and Aspergillus flavusspecies was found in 65 to 75% of moisture content were the pH 3 and pH 9 with the optimum temperature of 45°C.Among the different solid state substrates used nitrogen source of 1% peptone and 1% yeast extract accelerated the enzyme productionand it was also noted that 1% maltose and 1% starch showed higher enzyme production.The produced glucoamylase enzyme was finally eluted using the column chromatography. The eluted glucoamylase can be used for industrial product production like fermented foods.
