Beneficial bacterial organisms plays vital role in the life cycle of every plant, The PGPR organisms Gluconacetobacter diazotrophicus, Azospirillum brasilense and Bacillus megaterium associated with sugarcane were isolated from, five different locations namely Vilagam, Sivapuri, Vallampadugai, Erumpure and Killai, Cuddalore District, Tamil Nadu. In the present research two soil samples namely erumpure and killai showed zero percent population of Gluconacetobacter diazotrophicus, it shows the endphytic nature of Gluconacetobacter diazotrophicus in the absence of rich amount of organic matter content in soil.
