This article provides information about the work done for the method development and validation of combination of drugs like (Tiotropium Bromide and Salmeterol xinafoate), (Clarithromycin and Paracetamol), (Montelukast sodium and Fexofenadine hydrochloride) used in the pharmaceutical dosage forms. Similarlythe above work can also be done for herbal extracts like (Camellia sinensis, Phyllanthus emblica, Myristicamalabarica, Piper betle, Picrorhizakurroa) andthe results were recorded for different validation parameters such as Accuracy, Precision, Linearity, Robustness, Ruggedness, Retention time, % recovery, Limit of Detection(LOD), Limit of Quantification(LOQ) and Sensitivity by RP-HPLC method under suitable conditions like the type of column, pH, flow rate, mobile phase, and the detectors used.