The present study was conducted to determine antimicrobial activity of Calotropis procera against Escherichia coli and Proteus vulgaris. In plant parts leaves were chosen for this activity. The leaf extracts were prepared in three solvents: aqueous, petroleum ether and ethyl acetate. Concentration of plant extract 0.25-3mg/ml was used in study. The maximum zone of inhibition (20.3±0.93mm) was recorded in case of aqueous leaf extract of C. procera against Proteus vulgaris. Results revealed that MIC value was recorded for aqueous extract at 2.50 mg/ml and 2.75 mg/ml against E.coli and P.vulgaris respectively, whereas for ethyl acetate extract MIC was observed at 2.50mg/ml and 2.75 mg/ml for E.coli and P. vulgaris respectively. In case of petroleum ether MIC value was recorded at 2.50mg/ml and 2.75mg/ml for E.coli and P.vulgaris respectively.
