Bacteriocin producing lactic acid bacteria was isolated from cheese sample . The lactic acid bacterial strain was identified based on morphological, cultural,and biochemical characters and carbohydrate utilization pattern identified as Lactbacillus brevis LABC1. Antimicrobial activity of crude bacteriocin of Lactbacillus brevis LABC1 was tested against food borne bacterial pathogens such as Listeria monocytogenes MTCC 657 ,Bacillus cereus MTCC 1272, Escherichia coli MTCC 2939, Staphylococcus aureus MTCC 740, Salmonella typhi MTCC 531 . The methods used for testing antagonistic effect on the pathogen well diffusion assay ,Minimal inhibitory concentration, Minimal bactericidal concentration test. This study revealed that inhibition was obtained in 100µl in the well diffusion test. In MIC Among the food borne bacterial pathogens Listeria monocytogenes MTCC 657 recorded lower MIC (1.2ml) followed by staphylococcus aureus MTCC 740 (1.4ml) Bacillus cereus MTCC 1272 (1.6ml) , Escherichia coli MTCC 2939 (1.8 ml) and Salmonella typhi MTCC 531(2.0ml) whereas; In MBC Listeria monocytogenes MTCC 657 recorded lower MIC and MBC (1.8ml) followed by Staphylococcus aureus MTCC 740(2.0ml) Bacillus cereus MTCC 1272 (2.2ml) , Escherichia coli MTCC 2939 (2.4 ml) and Salmonella typhi MTCC 531(2.6ml) respctively.
