Biosurfactants are extracellular surface active, amphiphilic compounds produced by bacteria, fungi, and yeast which reduce surface and interfacial tension. The present study aimed at the isolation of biosurfactant producing bacteria and production of biosurfactant. The one isolated bacterium which is screened for highest production of biosurfactant was identified as Pseudomonas spp. based on biochemical and cultural characteristics. Pseudomonas spp. was used for biosurfactant production in Bushnell-Hass medium. The emulsification activity of extracted biosurfactant was tested against different vegetable oils and hydrocarbons and compared with chemical surfactant Triton X100 as a standard. The highest emulsification index [EI24] of biosurfactant was found against peanut oil and safflower oil i.e. 56.06 %.The extracted biosurfactant was characterized by FTIR analysis.The carbohydrate content was estimated at 46 µg/ml by using Anthrone method.
