Graphene is an allotrope of carbon consisting of a single layer of carbon atom arranged in an hexaGraphene oxidenal lattice. Graphene on conversion to Graphene oxide (GRAPHENE OXIDE) exhibits excellent physiochemical properties. GRAPHENE OXIDE can be prepared from the natural Graphite powder using chemical method, here GRAPHENE OXIDE was prepared using modified hummers method due its cost effectiveness. GRAPHENE OXIDE is rich with functional groups such as carboxyl (C=O), hydroxyl (-OH) and epoxide (Bridging Oxygen atom). The silver nanoparticles (AgNps) are synthesized from Catharanthus roseus and was incorporated into the prepared GRAPHENE OXIDE by incubation method and further loaded with phycocyanin. Phycocyanin is a light harvesting pigment isolated from Spirulina platensis and possess blue color which have a characteristic absorbance at visible light. The nanocomposite was further characterized using physiochemical methods such as UV-Vis Spectrophotometer, Fourier Transform Infra-Red (FT-IR) spectroscopy, X-ray powder diffraction (XRD) method and Scanning Electron Microscopy (SEM) study. From UV-Vis Spectroscopy study we observe that the peaks at 232nm(C=C) and a shoulder at 310nm(C=O) confirms the formation of GRAPHENE OXIDE. The GRAPHENE OXIDE was further confirmed by the FT-IR peak for carboxyl (C=O) at 1735cm-1. The XRD spectra of GRAPHENE OXIDE shows a sharp diffraction peak at 2θ=11.95 ̊ with an interlayer distance of 0.77nm which confirms the crystalline form of GRAPHENE OXIDE. The formation of phycocyanin coated GRAPHENE OXIDE-AgNps nanocomposite was confirmed by the presence of peaks at 232nm (GRAPHENE OXIDE), 420nm (AgNps) and 620nm (Phycocyanin) measured using UV-Vis Spectrophotometer. Thus prepared nanocomposite material was evaluated for its antibacterial and anticancer studies.
