DNA Barcoding reveals that identification of adult insects and parasitoids using cytochromeoxidase I gene and also provide molecular markers that may be used for simple rapid species identification. In the present study was carried out to identify the pumpkin beetles, A.foveicollis and A.nigripennis sp with use of molecular markers. The genomic DNA of A.foveicollis generated six bands (23130, 9416, 6557, 4361, 2322 and 2027 bp) and A. nigripennis the PCR product generated nine base pairs (10000, 6000, 3000, 2000, 1500, 1000, 750, 500 and 250 pb). The graphic summary shows the distribution of 100 BLAST hits on the query sequence with color key for alignment scores <40, 40-50, 50-80, 80-200 and > = 200. The alignments ranged from 1 to 658 and the number of matches and sequence ID-gb/KP898252.1. In A. nigripennis BLAST results showed the nucleotide sequence 647 letters,RID-SSYS870J101R, Query ID-209171 and the color key for alignment scores represents <40, 40-50, 50-80, 80-200 and >= 200. The alignment ranged 27-655 and the sequences are matched 86% for ID-gb/ KP 851141.4 in Aulacophora foveicollis (DNA sequence). The matching and mismatching of percentage may indicate the phylogenetic relationship and transformation of species level. The A. nigripennis sequences were not matched BLASTed against the gene bank public database (www.ncbi.nih.gov/blast). In this case (A. nigripennis) the present study had been the first to report sequence of A. nigripennis from chrysomelidae species.
