Reproduction in insects is an essential physiological process from the view point of propagation of the insect species. The testis, vas deference seminal vesicle and MARG of pumpkin beetles A. foveicollis (Lucas) and A.nigripennis (Motschulsky) were studied morphologically using scanning electron microscopy (SEM). In the present study morphological observations of testis, vas deference seminal vesicle and MARG of A. foveicollis and A. nigripennis the following surface observation have been examined. The testis were flattened leaf like structures, yellow coloured and surrounded by thick fibrous connective tissues and the entire structure with crystalline like layers of a peritoneal connective sheath. The testicular follicles with a number of pinocytotic pits, and itwas notable for its secretory function. Testicular tubes were covered with folded or ruffled connective tissues and covered with myoepithelial cells. The vas deferens of the A. foveicollis showed thick outer tubular structure with folded cuticle and myoepithelial cells. The epithelial layer consisted of numerous pinocytotic pits and secretory granules proved the epithelium to enhance its secretory activity. The seminal vesicle of the insect shows the tubular nature with outer ruffled muscles. The lumen contained more amount of secretory materials. The MARGs of the pumpkin beetle exhibit tubular structure with myoepithelialcells were covered with thin smooth cuticular muscles. The innervation of tracheae in the testes and accessory glands was observed in A.nigripennis. Testicular lobes of A.nigripennis were lined by a peritoneal layer an epithelial sheath, of the non-cellular layer (tunica propria). The vas deferens of A. nigripennis showed slender tube with externally covered by the smooth circular muscles with a number of secretory globules. The epithelial layer consisted of numerous pinocytotic pits and also secretory granules like that of A. foveicollis.The lumen of the seminal vesicle contained densely accumulated secretory materials with spermatozoa and also single layer of epithelial cells outside lumen. The seminal vesicle is externally covered by smooth circular and longitudinal muscle. The more morphological observations of testis, vas deference, seminal vesicle and MARG of A. foveicollis and A.nigripennisare helpful to trace the phylogenetic evolution of insect species.
